![]() Grey denotes additional sequence similarity between human and murine IB2. Three regions of IB2/IB1 similarity are blackened, and the percent amino acid sequence identities indicated. Human IB2 is aligned with the cloned segment of murine IB2 and with IB1. The lengths (in residues) of cores and flanking regions are indicated. Additional regions of sequence similarity among FHFs are in grey. The core homology region shared by FGFs and FHFs is denoted in black. Hence, FHFs are intracellular components of a tissue-specific protein kinase signalling module.įHF/FGF and IB2/IB1 sequence similarities. We further show that FHFs enable IB2 to recruit a specific MAPK in transfected cells, and our data suggest that the scaffolds IB1 and IB2 have different MAPK specificities. FHF/IB2 interaction is highly specific, as FHFs do not bind to the related scaffold protein IB1(JIP-1b), nor can FGF-1 bind to IB2. We show here that FHF-1 is associated with the MAP kinase (MAPK) scaffold protein Islet Brain-2 (IB2) in the brain and in specific cell lines. FGF-homologous factors (FHFs) were discovered within vertebrate DNA sequence databases by virtue of their sequence similarity to FGFs, but the mechanism of FHF action has not been reported. Fibroblast growth factors (FGFs) mediate cell growth, differentiation, migration, and morphogenesis by binding to the extracellular domain of cell surface receptors, triggering receptor tyrosine phosphorylation and signal transduction. ![]()
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